Abstract: Monthly average temperature at 29 meteorological stations in Huaihe River Basin during 1951-2014 was selected as subject. Multiple methods, including Sen trend analysis, Mann-Kendall test, anomaly analysis, cumulative anomaly analysis and spatial analysis, were applied to detect the characteristic of average temperature variation from temporal and spatial aspect. The results showed that (1) annual and seasonal average temperature had an increasing trend, and the annual and most seasonal series increased significantly except summer with slightly increasing. The changing temperature in the study region responded to global warming. (2) There was a sudden increasing change of spring and autumn temperature in 1990s as well as of winter temperature in 1980s, and a sudden decreasing change in 1960 is also detected for summer average temperature. (3) There was a decreasing trend of annual average temperature from south to north in Huaihe River Basin, and annual average temperature of entire basin has increased. (4) Spring and summer average temperature in the eastern Shandong and Jiangsu provinces (coastal region) was lower than that in the inland of the same latitude in Huaihe River Basin, and autumn and winter average temperature was higher due to the influence of ocean. (5) Annual and seasonal average temperature of the eastern Shandong and Jiangsu provinces increased remarkably, which was connected with decreasing annual precipitation. In Zhengzhou and Kaifeng areas, annual average temperature had increased, which was probably related to local human activities.