荧光法研究阿托伐他汀钙与牛血清白蛋白的相互作用

Interaction of Atorvastatin Calcium with Bovine Serum Albumin by Fluorescence

摘要: 在不同温度下测定了阿托伐他汀钙(AC)对牛血清白蛋白(BSA)的荧光光谱的影响.结果表明,AC会使BSA发生荧光猝灭,随着AC浓度的增大,猝灭程度增强.AC对BSA的猝灭作用主要是动态猝灭,并计算得到AC与BSA的结合位点数为1.2.根据热力学方程式得出AC与BSA相互作用的参数,说明AC与BSA之间的相互作用力主要是疏水作用力.

Abstract: The interaction between atorvastatin calcium(AC) and bovine serum albumin (BSA) was studied by fluorescence spectrum at different temperatures.The results showed that the fluorescence quenching of BSA was caused by AC. The degree of quenching was enhanced with the increase of the concentration of AC. The quenching belonged to dynamic quenching mostly. The binding sites of the AC and BSA was 1.2.The parameters of the interaction between AC and BSA were obtained according to the thermodynamic equation. The results indicated that the interaction between AC and BSA was mainly hydrophobic force.