Abstract: A new barbine fish, Sinocyclocheilus punctatus sp. nov. has been described based on five specimens collected from Nandan and Huanjiang Counties, Guangxi, China. Sinocyclocheilus punctatus is distinguished from other species of Sinocyclocheilus by having the following combination of characters:dorsal fin with 8 branched rays, last unbranched dorsal-fin ray strong with serrations on posterior edge of its lower part; pelvic-fin origin anterior to dorsal-fin origin; mouth terminal; pectoral fin long, surpassing pelvic-fin origin; rostral barbels short, can not reach anterior edge of eyes; body covered with minute scales, and lateral line with 45~50 scales, lateral line scales are bigger than their neighbor scales; outer face of first arch with 7~8 gill rakers; dorsal surface of head, dorsum and body side above lateral line covered with black spots.