Abstract: The HeLa cells was co-infected by the retrovirus which carried these four kinds of transcription factors and was cultivated on MEF after infection. After that, the infected cells generated a clone group that can be amplified, and successfully differentiated into neurons in a similar way as embryonic stem cells do. The expression of Pax6, Sox1 and βⅢ-tubulin can be detected by immunofluorescence after different days of differentiation. Finally, the results showed that HeLa cells can be successfully reprogrammed to iPS cells under the influence of these four kinds of transcription factors and also can further differentiate into neurons.