Abstract: Gold-aspartic acid composite film modified electrode  was fabricated via a layer by layer technique. The electrochemical properties of uric acid (UA) on the sensor were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results demonstrated that the oxidation peak current  of UA on the sensor was 6.5 times than that of bare electrode when PBS 7.0 acting as the supporting electrolyte. Under optimum conditions, the oxidation peak current of UA had a linear relationship as a function of its concentration ranging from 4.0×10-7 to 1.0×10-4  mol/L. The linear equation wasI (μA) = 0.010 - 0.022 C (μmol/L). The correlation coefficient and detection limit (RSN=3) were 0.998 and 1.0×10-7 mol/L, respectively. The recovery efficiency was 99.4% ～ 104.1% when the method was used for actual urine sample detection.