Abstract: An ASE-UPLC method for simultaneous determination of three components including geniposidic acid, chlorogenic acid and pinoresinol diglucoside in the different preparations containing Eucommia ulmoides was established. The ASE extraction was conducted with 70% MeOH-H2O as solvent at 40 ℃ and 103.4 MPa, and UPLC analysis was performed on an Acquity UPLC BEH C18 column (100 nm×2.1 mm,1.7 μm) with acetonitrile (A)-0.1% formic acid (B) as mobile phases for gradient elution (0~15 min, 70% B~90% B) at the flow rate of 0.15 mL/min. The detection wavelength was 237 nm and the column temperature was 20 ℃. The results showed that three components were separated completely and showed good linearity, precision and repeatability (RSD<3.0% respectively). Recoveries were between 95.84%~102.69% (RSD<3.0%). The data suggested the established method was rapid, accurate, good reproducible and could be applied to simultaneously determine main components in the preparations containing Eucommia ulmoides.