Abstract: The poly crystal violet film modified glassy carbon electrode was prepared by electrochemical polymerization method.The electrochemical behaviors of quercetin on the modified electrode were studied by cyclic voltammetry and differential pulse voltammetry  in phosphate buffer.The results showed that the poly crystal violet film could increase the electrochemical response of quercetin on electrode. Under optimized conditions, the oxidation peak current of quercetin had good linear relationship with its concentration in a wide range of 5.0×10-6~1.5×10-4 mol/L with a related coefficient (R) of 0.995 and detection limit of 8.0×10-7 mol/L (RSN=3). The method was used to detect rutin quercetin in hydrolysate ,the recoveries were 102. 8%～104. 3%,and RSD＜2.5%.