Abstract: The interaction between dandelion extract and bovine serum albumin (BSA) was investigated by fluorescence spectrum. The effects of the different concentrations of dandelion extract and the kinds of solvent on the fluorescence intensity were discussed. The experimental results showed that the fluorescence intensity of BSA is enhanced as a result of the dandelion flowers or leaves of methanol extract. The biggest fluorescence emission wavelength had no obvious red shift. Fluorescence quenching of BSA was produced due to the ethanol extracts of dandelion flowers or leaves, and its biggest fluorescence emission wavelength had obvious red shift. With the extract concentration increases, △F of  the dandelion methanol extracts of interaction with BSA had linearly increasing trend, while △F of the dandelion ethanol extract of interaction with BSA had a downward trend.