Abstract: Molybdenum disulfide (MoS₂)-carbon nanoparticle (CNP) composites were synthesized by a modified L-cysteine assisted solution-phase method and the morphologies were characterized by scanning electron microscopy (SEM). MoS₂-CNP composites modified glassy carbon electrode (GCE) was constructed as a sensitive electrochemical sensor for the detection of acetaminophen. The electrochemical behaviors of acetaminophen were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results revealed that the electrochemical sensor exhibited a linear range of 0.1~100 μmol/L and detection limit of 0.01 μmol/L. Furthermore, this method was successfully applied to determine acetaminophen in pharmaceutical samples. The sensor showed high sensitivity, stability, anti-interference ability and good reproducibility