基于酪氨酸酶/ 离子液体 - 石墨烯纳米复合界面的双酚 A 电化学传感器 解放军防空兵学院,河南 郑州450000

基于酪氨酸酶/ 离子液体 - 石墨烯纳米复合界面的双酚 A 电化学传感器解放军防空兵学院,河南郑州450000

摘要: 以 1 , 3 - 二( 4 - 氨基 - 吡啶) 丙烷四氟硼酸盐离子液体修饰石墨烯纳米复合物, 负载酪氨酸酶至玻碳电极表面构建双酚 A 电化学传感器 . 优化了滴涂量、检测电位、p H值等实验条件对传感器响应性能的影响 . 结果表明, 传感器的安培响应电流与双酚 A 浓度在 1.0×1 0-9 ~3.8×1 0-5m o l / L 范围内具有良好的线性关系,检出限为 3.5×1 0-1 0m o l / L ( R S N =3 ) . 用于矿泉水瓶中溶出双酚 A 含量测定, 结果与高效液相色谱一致 .

Abstract: Graphene-1,3-di(4-amino-1-pyridinium) propane tetrafluoroborate ionic liquid nanocomposite (Gr-DAPPT) modified on a glassy carbon electrode and then tyrosinase (Tyr) was immobilized onto the electrode to fabricate a biosensor. Experimental conditions including the volume of Tyr-Gr-DAPPT solution, the applied potential, and pH value were optimized for bisphenol A (BPA) determination. The biosensor exhibited a linear response to BPA in the concentration range from 1.0×10^-9to 3.8×10^-5mol/L. The detection limit was calculated to be 3.5×10^-10mol/L (R_SN=3). Practical application of the biosensor was demonstrated by determining BPA leaching from commercial plastic drinking bottles. The result was consistent with that obtained by high performance liquid chromatography