Abstract: Parallel factor method were used to analyze fluorescence excitation-emission matrix spectra of dissolve organic matter which was from 31 lakes’ sediment pore water in Hubei province, and the clustering analysis was carried out on the fluorescence components to investigate its lake basins’ clustering phenomenon. The results showed that: (1) The fluorescence spectra of all the samples had a similar shape and peak position, the main fluorescence peaks were humic-like substance and protein-like substance. But we saw that the migrating and overlapping phenomenon existed in the fluorescence peak positions. (2) The fluorescence spectra was divided into five independent, they were fulvic-, humic-, tryptophan- and tyrosine-like acid, and another fulvic-like acid separated because of the interactions between the tyrosine-like and fulvic-like components，respectively. (3) In the analysis for relative content of fluorescence components, like acid, whose total content was higher than that of humic- and protein-like substance, was more active organic acid in the studied lakes’ sediment. (4) From the clustering results, the difference between the fluorescence components of DOM in lakes’ sediment pore water was not obvious; there were only small differences between the lake basins