高效液相色谱法测定磷酸肌酸钠及其有关物质
Determination of Sodium Phosphocreatine and its Related Substances by HPLC
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摘要: 采用高效液相色谱法测定磷酸肌酸钠及其有关物质的含量,色谱柱为phenomenex Luna HILIC,乙腈-20 mmol·L-1磷酸氢二铵(pH为7.0)(77∶23)为流动相,流速为0.8 mL·min-1,检测波长为210 nm,柱温为35℃.结果表明:磷酸肌酸钠与其有关物质肌酸、肌酐在同一检测条件下能够完全分离,磷酸肌酸钠、肌酸、肌酐分别在0.06~2.24(r=0.999 9)g·L-1、0.05~2.10(r=0.999 9)g·L-1和0.05~2.04(r=0.999 9)g·L-1范围内浓度与峰面积呈良好线性关系,其平均回收率分别为磷酸肌酸钠97.8%,肌酸100.4%,肌酐96.1%,RSD分别为1.5%、1.3%、0.7%.该方法操作简便,灵敏度高,专属性强,可用于磷酸肌酸钠及其有关物质的测定Abstract: A method for determination of Sodium phosphocreatine and its related substances was established by HPLC method. The determination was performed on phenomenex Luna HILIC column, acetonitrile20 mmol/L diammonium phosphate solution (pH 7.0) (77∶23) as the mobile phase at a flow rate 0.8 mL/min.The detection wavelength was 210 nm and the column temperature was 35 ℃. The results showed that sodium phosphocreatine and its related substances could be completely separated under the same test conditions.Good linear relationship between the peak areas and the concentrations of sodium phosphocreatine, creatine and creatinine were 0.06~2.24 (r=0.999 9) g/L, 0.05~2.10 (r=0.999 9) g/L, and 0.05~2.04 (r=0.999 9) g/L, respectively.The average recoveries were 97.8%(RSD=1.5%), 100.4%(RSD=1.3%), 96.1%(RSD=0.7%) respectively. The method was simple, high sensitive, good specialization, and could be used to determine sodium phosphocreatine and related substances.
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