Abstract: Human salivary gland adenoid cystic carcinoma(ACC-M) cells in vitro were treated by using 0 (control group), 5, 10, 20, 40, 80 μmol·L^-1 icotinib hydrochloride,respectively ,and the morphology change of ACCM cells were observed after 12, 24, 48, 72, 96 h. Using methyl thiazolyl tetrazolium (MTT method) to test the inhibition rate of ACCM cells effected by different concentrations of icotinib hydrochloride in different time span. According to the results, the ACC-M cells become smaller, with the cytoplasmic shriveled, intercellular space expanded, intracellular particles increased, and adherent cells turning round, floating. Icotinib hydrochloride restrains the proliferation of ACCM cells.