Abstract: The effect of allicin on PKC in exercise-induced fatigue rats was investigated. SD rats aged 8 weeks were randomly divided into 3 groups: 1) Control, 2) Training, and 3) Training + Allicin. Each group was further randomly divided into two sub-groups, according to the two time of sampling: 1) 24 h post training and 2) 72h post training. Rats in Training and Training + Allicin had been performed incremental load swimming training for 5 weeks. The concentrations of PPKC collected from spleen lymphocytes were measured. The concentrations of P-PKC collected from spleen lymphocytes in Training (P<0.01) and Training + Allicin (P<0.05) were both significantly lower than that in Control. While which in Training + Allicin showed no significant difference compared with that in Training. Exerciseinduced fatigue may be associated with the change of P-PKC. Therefore, allicin had no significant effect on the change of P-PKC.