Abstract: Tetra-(p-methoxyphenyl) porphyrin nickel complex was synthesized.The binding reaction between tetra(p-methoxyphenyl) porphyrin Ni(Ⅱ) complex and bovine serum albumin(BSA) was investigated by fluorescence and UV-vis absorption spectrum.The fluorescence intensity of BSA was quenched when tetra-(p-methoxyphenyl) porphyrin Ni(Ⅱ) complex was added. Using Stern-Volmer equation and doublereciprocal equation,the quenching constant and binding constant were calculated to be: K_q=3.09×10¹² L·mol^-1·s^-1,K_A = 2.62×10⁴ L·mol^-1, n=0.991. And the results also showed that the quenching was static quenching.