Abstract: The hollow PtCu nanocomposite has a large specific surface area and good permeability due to the interior hollow structure and rough porous surface, which can improve the electron transfer rate and oxidation peak current of 2-hydroxyanthraquinone effectively. An electrochemical sensing platform with high selectivity and sensitivity is developed for the fast determination of 2-hydroxyanthraquinone. The electrochemical oxidation behaviors of 2-hydroxyanthraquinone are investigated by cyclic voltammetry and differential pulse voltammetry. The electrolyte, amount of modifier, and accumulation conditions are optimized. Under the optimal conditions, the linear range is 0.80 nmol/L~50 μmol/L and the limit of detection is 0.60 nmol/L (R_SN=3). The method is successfully applied to detect 2-hydroxyanthraquinone in environmental samples.