Abstract: Triple hydrogen bonded supramolecular self-assembly systems are widely used in the fields of molecular recognition, catalyst, soft materials and functional materials, due to their several advantages, such as strong binding power, simple synthesis, structure can be modified easily, predictable recognition performance, and so on. In this review, recent developments of triple hydrogen bonded systems are summarized and their application prospects are discussed with 65 references. More specifically, the design principles and the factors affecting the stability of triple hydrogen bonded systems are emphatically elaborated. Based on this, the research directions of triple hydrogen bonded supramolecular self-assembly systems in the future are prospected, including the design and synthesis of stable triple hydrogen-bonded units under physiological conditions and the further study of assembly mechanism.