Abstract: Regular catching method was employed to study diversity of fly species in Wuhan area from January 2006 to December 2016. A total of 15 603 flies were captured, belonging to 11 species under 7 genera and 4 families and Musca domestica and Lucilia sericata were dominant species. Flies were active annually, and the total density seasonal fluctuation had two peaks, with the first peak in May and the second peak in September. The peaks of fly density were different as a result of the high density from April to November in different years. Except for 2010(14.5/cage), the overall trend of the inter annual fluctuation declined gradually. Followed by residential areas, the richness index, diversity index and evenness index of flies in greenbelt were the highest, and outside of restaurants and marketplaces were the lowest. The total similarity indexes of four habitats in the fly community ranged from 0.87 to 1.00, which were extremely similar. The hierarchical cluster and the non-metric multidimentional scaling(MDS) method based on euclidean distance showed that the date of 2006-2008, 2010 were gouped together, and the date of 2011-2016 were grouped together, which were close to each other. These results indicated that the diversity of flies in the four habitats was closely related to climate and human disturbance factors.