Abstract: Aimed to improve the economic efficiency of wheat stalk and get high value-added chemicals,bio-oil,prepared from the liquefaction of wheat stalk in supercritical ethanol at 280℃,was first extracted by benzene.The benzene-soluble fraction was extracted by ether before and after acid-base neutralization.The residues were future separated by column chromatography.All the separated fractions were analyzed by GC/MS.Results showed that hydroxy-ketones and phenolic compounds were effectively separated by the extraction of ether,and the residues composition was much more complex.Through the separation of column chromatography,components such as C₈~C₃₀ alkanes,naphthalene and its derivatives,and alkyl cyclopentenones were enriched and separated.What's more,nitrogen compounds were also enriched and detected by GC/MS,which was helpful to full the composition information of bio-oil.