Abstract: Non-coding RNA Bm-152 accumulated more in silk gland of silkworm Bombyx mori. Real-time PCR was used to study the expression levels of Bm-152 in the silk glands of silkworm from the fifth instar larvae to prepupa stage. Furthermore, the signaling pathway in which Bm-152 may participate were studied as Bm-152 was overexpressed in the 3^rd day of the fifth larva of silkworm. Results showed that Bm-152 had no obvious change during the development of silk glands of the fifth instar larvae of silkworm, Bm-152 had more expression at the first spinning day in the silk gland, then decreased in the pre-pupa stage. After 48h injection of 2μg dose of piggyBacA3-EGFP-A3-Bm-152 overexpression vector, it was found that Bm-152 was not overexpressed in the silk gland, but was significantly overexpressed in the non-silk gland, and the expression of Bm-152 increased 1.66 times. Moreover, the expression of Usp (ecdysone signaling pathway) increased by 1.95 times in the non-silkgland tissues, E75A (ecdysone signaling pathway) increased by 2.26 times, and the expression of Met(juvenile hormone signaling pathway)increased by 1.79 times in the non-silkgland tissues.Bm-152 might participate in the development of silkworm through the ecdysone and juvenile hormone signaling pathway. These results laid foundations for further study of functions of Bm-152 in silkworm.