Abstract: Dukouhe Bridge is a goods and passenger railway bridge whose piers was once highest in the word. In order to verify the correctness of the design theory, and to provide the scientific evidence for the acceptance of this bridge, the static and dynamic loading tests were made. The testing subject was 72 m+128 m+72 m continuous steel structures of a half span structure in Yi-Chang port. Based on the structure of Dukouhe crossing bridge, the testing contents contained the deformation of the bridge; the stress state of the primary members; the natural frequency; the displacement, acceleration and the amplitude of the bridge under the different velocities and the emergency braking. The test results showed that: the design theory was right, the quality of the bridge manufacture and construction was good and the working state of the bridge satisfied the design demands. The test provided some technological parameters for the safe operation later, and accumulated some important technological materials of the static and dynamic characters of the bigger spacing and more complicated bridges