Abstract: The scutellarin of compound lobelia injection was extracted by the intergrated treatment with α-amylase and ultrasonic,and was determined by HPLC in orthogonal experiment.The optimal conditions were the following: the enzymolysis time is 30 min, the optimal pH is 6.0,the enzymolysis temperature is 30 ℃ with the α-amylase dosage is 0.5 g . Under such optimized conditions, the content of scutellarin in compound lobelia injection is 4.45 g/L, which is significantly more than 1.34 g/L through decocting technology contained in registration standards,3.84 g/L through single ultrasonic extraction, and 1.89 g/L trough single α-amylase extraction.