Abstract: The porcine hnRNPK gene promoter were cloned firstly and analysis by the bioinformatics software. The results showed that the length of porcine hnRNPK promoter is about 1 kb, and had 78.9 percent of the similarity comparing with human hnRNPK promoter, which had the same core promoters’ sequence of “TCTCGCGAGA” and transcriptional start site. The results of bioinformatics analysis showed that no TATA box and two CpG island are found in porcine hnRNPK gene promoter region, and exited many transcription factorsbinding sites such as SP1, UCE.2、GCF, EARLYSEQ1, TTR_inverted_repeat, NGFIC, and 8 motifs.