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李先文, 李铃, 李铮铮, 张新玉. 组成型表达CsCOR1基因烟草的制备[J]. 信阳师范学院学报(自然科学版), 2012, 25(1): 64-66.
引用本文: 李先文, 李铃, 李铮铮, 张新玉. 组成型表达CsCOR1基因烟草的制备[J]. 信阳师范学院学报(自然科学版), 2012, 25(1): 64-66.
shu
LI Xian-wen, LI Ling, LI Zheng-zheng, ZHANG Xin-yu. Preparation of Tobacco Transformants with Overexpression CsCOR1 Gene[J]. Journal of Xinyang Normal University (Natural Science Edition), 2012, 25(1): 64-66.
Citation: LI Xian-wen, LI Ling, LI Zheng-zheng, ZHANG Xin-yu. Preparation of Tobacco Transformants with Overexpression CsCOR1 Gene[J]. Journal of Xinyang Normal University (Natural Science Edition), 2012, 25(1): 64-66.
shu

组成型表达CsCOR1基因烟草的制备

Preparation of Tobacco Transformants with Overexpression CsCOR1 Gene

    摘要
  • 摘要: 将已克隆的一个被冷显著上调的基因CsCOR1整合到穿梭载体pBI121的CaMV35S启动子的下游,然后采用农杆菌介导法转化进烟草细胞,从而制得了转CsCOR1基因烟草植株,并筛选到组成型表达CsCOR1基因的烟草转化子,为进一步研究该基因的功能奠定了基础.

     

    Abstract: CsCOR1,a gene upregulated remarkably by cold,was inserted CaMV35S promoter behind of pBI121 vector,producing a pBI121-CsCOR1.Then,the vector was introduced Agrobacterium tumefaciens strain EHA105 using freeze-thaw transformation.Transgenic-tobaccos were generated by Agrobacterium-mediated transformation using the leaf disc system,and two plants overexpressing CsCOR1 gene were screened.The harvests lay the foundation of CsCOR1 function research

     

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