番红褪色法检测羟自由基清除效果研究

The Method of Detecting Hydroxyl Radical Scavenged by Faded Safranine

摘要: 根据Fenton反应原理,利用番红作为显色剂检测由EDTANa2-Fe(Ⅱ)-H2O2体系产生的.OH.番红褪色光度法测定清除羟自由基的最佳反应条件为:往10 mL具塞试管中,依次加入pH7.4的磷酸缓冲液1.0mL,番红溶液(520 mg/L)0.2 mL,EDTANa2-Fe(Ⅱ)(0.003 8 mol/L)1.0 mL,不同浓度的样品溶液0.1mL,最后加入0.25%H2O20.5 mL,双蒸水稀释至刻度后于40℃保温30 min,紫外-可见分光光度法519 nm处测吸光度A.

Abstract: Based on the priciple of Fenton reaction,the ·OH produced by the EDTANa2-Fe(Ⅱ)-H2O2 system can be detected by using safranine as developer.The optimal experimental conditions is evaluated: 1.0 mL pH7.4 phosphate buffer;safranine(520 mg/L) 0.2 mL,EDTANa2-Fe(Ⅱ)(0.003 8 mol/L) 1.0 mL,and 0.25% H2O2 0.5 mL.All these agent must be added one by one into the 10 mL capped test tube.Redistilled water was diluted to graduation line,then incubated at 40 ℃.The amount of hydroxyl radical produced in the system can be in...