刘希宇, 贺小英, 王凤梅, 程腾, 马利兵. 石蒜碱对人胃癌细胞体外增殖的影响[J]. 信阳师范学院学报(自然科学版), 2015, 28(2): 173-176. DOI: 10.3969/j.issn.1003-0972.2015.02.005
引用本文: 刘希宇, 贺小英, 王凤梅, 程腾, 马利兵. 石蒜碱对人胃癌细胞体外增殖的影响[J]. 信阳师范学院学报(自然科学版), 2015, 28(2): 173-176. DOI: 10.3969/j.issn.1003-0972.2015.02.005
Liu Xiyu , He Xiaoying , Wang Fengmei , Cheng Teng , Ma Libing . The Influence of Lycorisradiata Alkali on the Proliferation of Human Gastric Cancer Cells Cultured in Vitro[J]. Journal of Xinyang Normal University (Natural Science Edition), 2015, 28(2): 173-176. DOI: 10.3969/j.issn.1003-0972.2015.02.005
Citation: Liu Xiyu , He Xiaoying , Wang Fengmei , Cheng Teng , Ma Libing . The Influence of Lycorisradiata Alkali on the Proliferation of Human Gastric Cancer Cells Cultured in Vitro[J]. Journal of Xinyang Normal University (Natural Science Edition), 2015, 28(2): 173-176. DOI: 10.3969/j.issn.1003-0972.2015.02.005

石蒜碱对人胃癌细胞体外增殖的影响

The Influence of Lycorisradiata Alkali on the Proliferation of Human Gastric Cancer Cells Cultured in Vitro

  • 摘要: 以体外培养的hGCCs MGC-803细胞系为研究对象,采用不同浓度石蒜碱(5、2.5、1.25 μmol/L)进行处理,倒置显微镜下观察其生长状态,同时采用血球计数板统计其体外增殖率,采用流式细胞技术检测处于不同细胞周期的细胞比例.结果表明,石蒜碱处理对hGCCs的体外增殖具有一定的抑制作用,其中5 μmol/L石蒜碱对hGCCs的抑制作用最为明显.显微观察和计数分析结果表明,5 μmol/L石蒜碱处理的hGCCs活力最低,细胞凋亡现象最明显.细胞周期检测的结果显示石蒜碱处理会使hGCCs增殖趋缓,细胞周期延长

     

    Abstract: The hGCCs were treated with different concentration of lycorisradiata alkali (5、2.5、1.25 μmol/L respectively), growth status were observed by the inverted microscope, and proliferation rate were count by hemocytometer, the proportion of hGCCs in different cell cycles were detected by flow cytometry. The results showed that the proliferation of hGCCs was suppressed by treated with lycorisradiata alkali, the most obvious suppressed of hGCCs was 5 μmol/L treatment group. Through microscopic observation and counting analysis results, it has been found that the vitality of hGCCs was the lowest and the cells apoptosis was the most obvious by treated of 5 μmol/L lycorisradiata alkali. The result of cell cycle detection revealed that by treated with lycorisradiata alkali, the reproduction of hGCCs became slow and cell cycle became extended

     

/

返回文章
返回