周棋赢, 韩月华, 梁萍, 王艳丽, 穆逸雪, 王仪佳, 祝悦, 刘国峰, 彭波, 袁红雨. 茶树NAC基因的鉴定及其在逆境反应中的表达调控分析[J]. 信阳师范学院学报(自然科学版), 2020, 33(4): 567-578. DOI: 10.3969/j.issn.1003-0972.2020.04.010
引用本文: 周棋赢, 韩月华, 梁萍, 王艳丽, 穆逸雪, 王仪佳, 祝悦, 刘国峰, 彭波, 袁红雨. 茶树NAC基因的鉴定及其在逆境反应中的表达调控分析[J]. 信阳师范学院学报(自然科学版), 2020, 33(4): 567-578. DOI: 10.3969/j.issn.1003-0972.2020.04.010
ZHOU Qiying, HAN Yuehua, LIANG Ping, WANG Yanli, MU Yixue, WANG Yijia, ZHU Yue, LIU Guofeng, PENG Bo, YUAN Hongyu. Genome-wide Identification of NAC Genes in Tea Plant and Its Expression Regulation in Stress Response[J]. Journal of Xinyang Normal University (Natural Science Edition), 2020, 33(4): 567-578. DOI: 10.3969/j.issn.1003-0972.2020.04.010
Citation: ZHOU Qiying, HAN Yuehua, LIANG Ping, WANG Yanli, MU Yixue, WANG Yijia, ZHU Yue, LIU Guofeng, PENG Bo, YUAN Hongyu. Genome-wide Identification of NAC Genes in Tea Plant and Its Expression Regulation in Stress Response[J]. Journal of Xinyang Normal University (Natural Science Edition), 2020, 33(4): 567-578. DOI: 10.3969/j.issn.1003-0972.2020.04.010

茶树NAC基因的鉴定及其在逆境反应中的表达调控分析

Genome-wide Identification of NAC Genes in Tea Plant and Its Expression Regulation in Stress Response

  • 摘要: 利用隐马可夫模型检索和同源比对的方法,从茶树基因组鉴定出108个NAC成员(CsNAC).理化特征、进化关系、基因结构和保守基序分析表明,CsNAC蛋白长度在134~1950个氨基酸之间,分子量介于15.4~222 kD,等电点介于4.60~9.91,大部分为亲水性蛋白(除CsNAC97).其中,CsNAC3、8、14、23、32、74、82、90、106蛋白含跨膜结构域.进化分析将CsNAC家族分为18个亚类,ONAC022和NAP亚类成员最多,AtNAC3和OsNAC8亚类成员最少.CsNAC基因启动子区富含响应干旱、低温、盐胁迫以及病原菌侵染的序列元件.据转录组数据分析结果,CsNAC基因家族成员在不同组织和胁迫处理后呈现显著的表达差异.低温和干旱胁迫下的CsNAC共表达网络中,ABA参与调控其关键基因的表达;KEGG分析显示,信号传递、糖代谢、植物与病原菌互作等途径存在显著富集.

     

    Abstract: By using the Hidden-Markov model searching and homologous alignment methods, 108 NAC members (CsNAC) were identified in tea plant genome.For all CsNAC members,physicochemical parameters, evolutionary relationships, gene structures, and conserved motifs were investigated.Results showed that the amino acid size, molecular weight and isoelectric point of CsNAC proteins varying from 134~1950 a.a., 15.4~222 kD, and 4.60~9.91, respectively, and CsNAC3,8,14,23,32,74,82,90 and 106 had transmembrane domains.The grand average of hydropathy value indicated that all CsNAC proteins were water soluble, except the CsNAC97 protein. CsNAC family was divided into 18 subclasses by evolutionary relationship analysis.ONAC022 and NAP subclasses had the most members, while AtNAC3 and OsNAC8 had the fewest members. The promoter region of CsNAC gene is rich in cis-elements in response to drought, low temperature, salt stress and pathogen infection.Transcriptomic data analysis showed that CsNAC gene family members displayed significant differential expression patterns in different organs of tea plants and under various stress treatments.Under cold and drought stresses,ABA involved in regulating the expression of key genes in the CsNAC co-expression network. KEGG analysis of genes in the co-expression network revealed that pathways as signal transduction, carbonhydrates metabolism, plant-pathogen interaction, etc, were significantly enriched.

     

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