陈磊, 李开鸣, 马会景, 王芳, 曾宪思, 宋新强. 三维培养体系下EGCG对乳腺癌干细胞迁移性和自我更新的抑制效应[J]. 信阳师范学院学报(自然科学版), 2019, 32(3): 437-442. DOI: 10.3969/j.issn.1003-0972.2019.03.017
引用本文: 陈磊, 李开鸣, 马会景, 王芳, 曾宪思, 宋新强. 三维培养体系下EGCG对乳腺癌干细胞迁移性和自我更新的抑制效应[J]. 信阳师范学院学报(自然科学版), 2019, 32(3): 437-442. DOI: 10.3969/j.issn.1003-0972.2019.03.017
CHEN Lei, LI Kaiming, MA Huijing, WANG Fang, ZENG Xiansi, SONG Xinqiang. The Inhibitory Effect of EGCG on Breast Cancer Stem Cell Migration and Self-renewal Properties in 3D Culture System[J]. Journal of Xinyang Normal University (Natural Science Edition), 2019, 32(3): 437-442. DOI: 10.3969/j.issn.1003-0972.2019.03.017
Citation: CHEN Lei, LI Kaiming, MA Huijing, WANG Fang, ZENG Xiansi, SONG Xinqiang. The Inhibitory Effect of EGCG on Breast Cancer Stem Cell Migration and Self-renewal Properties in 3D Culture System[J]. Journal of Xinyang Normal University (Natural Science Edition), 2019, 32(3): 437-442. DOI: 10.3969/j.issn.1003-0972.2019.03.017

三维培养体系下EGCG对乳腺癌干细胞迁移性和自我更新的抑制效应

The Inhibitory Effect of EGCG on Breast Cancer Stem Cell Migration and Self-renewal Properties in 3D Culture System

  • 摘要: 以乳腺癌细胞三维培养体系为基础,探究(-)-表没食子儿茶素-3-没食子酸酯((-)-Epigallocatechin-3-gallate,EGCG)对乳腺癌干细胞(BCSCs)的药理学作用.细胞迁移性实验结果发现,20和80 μmol/L EGCG能显著抑制迁移性基因的转录水平,降低通过Transwell穿膜细胞的数量,延缓癌细胞划痕修复时间,抑制癌细胞迁移性.在对BCSCs自我更新研究中,EGCG通过下调干细胞关键基因的转录而抑制癌细胞单克隆形成和克隆形成能力,抑制癌细胞自我更新,并有效降低CD24-亚群BCSCs含量.研究结果表明,在三维培养条件下,EGCG对BCSCs的迁移性和自我更新具备显著的抑制效应.

     

    Abstract: The pharmacological effects of (-)-epigallocatechin-3-gallate (EGCG) on BCSCs were investigated in 3D culture system. The transcription level of epithelial-mesenchymal transition (EMT) associated genes were significantly downregulated by EGCG, resulting in the inhibition of BCSC migration including the reduction of cell number through 80 μmol/L pore and the attenuation of scratch repair capacity. In addition, the self-renewal capabilities including the expression level of stem cell marker genes and the clone formation capacity were also inhibited in cells cultured in 3D by EGCG. The proportion of stem cell population as detected by flow cytometry with CD24-/low phenotype was also reduced by 80 μmol/L EGCG. Overall, the migration and self-renewal properties of BCSCs were significantly inhibited by EGCG in cancer cells cultured in 3D.

     

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