王海波, 董高丽, 白宏宇, 李阳, 毛安丽. 基于BSA模板荧光铜纳米簇的生物传感方法灵敏检测四环素[J]. 信阳师范学院学报(自然科学版), 2018, 31(4): 632-635. DOI: 10.3969/j.issn.1003-0972.2018.04.022
引用本文: 王海波, 董高丽, 白宏宇, 李阳, 毛安丽. 基于BSA模板荧光铜纳米簇的生物传感方法灵敏检测四环素[J]. 信阳师范学院学报(自然科学版), 2018, 31(4): 632-635. DOI: 10.3969/j.issn.1003-0972.2018.04.022
WANG Haibo, DONG Gaoli, BAI Hongyu, LI Yang, MAO Anli. Fluorescence Sensing Strategy for Sensitive Detection of Tetracyclines Based on Bovine Serum Albumin Templated Copper Nanoclusters[J]. Journal of Xinyang Normal University (Natural Science Edition), 2018, 31(4): 632-635. DOI: 10.3969/j.issn.1003-0972.2018.04.022
Citation: WANG Haibo, DONG Gaoli, BAI Hongyu, LI Yang, MAO Anli. Fluorescence Sensing Strategy for Sensitive Detection of Tetracyclines Based on Bovine Serum Albumin Templated Copper Nanoclusters[J]. Journal of Xinyang Normal University (Natural Science Edition), 2018, 31(4): 632-635. DOI: 10.3969/j.issn.1003-0972.2018.04.022

基于BSA模板荧光铜纳米簇的生物传感方法灵敏检测四环素

Fluorescence Sensing Strategy for Sensitive Detection of Tetracyclines Based on Bovine Serum Albumin Templated Copper Nanoclusters

  • 摘要: 利用牛血清白蛋白(BSA)模板荧光铜纳米簇为荧光探针,发展了一种快速、非标记型的荧光生物传感新方法用于四环素的灵敏检测.结果表明,四环素的线性检测范围为5.0×10-8~4.0×10-5 mol/L,检出限为5.0×10-9 mol/L (RSN=3).该方法已成功地应用于牛奶样品中四环素含量的测定,相对误差不大于3.3%,加标回收率在96.0%~102.8%之间.该研究为四环素的灵敏检测提供了一种快速、方便的新方法.

     

    Abstract: A rapid and label-free fluorescent sensing method has been established for sensitive detection of tetracycline (TC) by using bovine serum albumin (BSA) templated CuNCs as fluorescence probes. The strategy permitted sensitive detection of tetracycline in a linear range from 5.0×10-8 to 4.0×10-5 mol/L and with a detection limit of 5.0×10-9 mol/L. The sensor was successfully applied to determinate the tetracycline content in milk samples. It could provide a rapid and convenient fluorescent platform for tetracycline detection.

     

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