Abstract: The 174 adults (103 males, 71 females) of Han ethnic aged 18 to 60 yeas were studied and they were grouped by gender and major respectively. The length of index finger (2D), middle finger (3D), ring finger (4D) and little finger (5D) were measured and the corresponding digit ratio was calculated. SPSS 23.0 was used for data analysis.The t test and multi-way ANOVA were used to compare the group differences.The results showed:(1) When grouped by gender, there were significant differences between men and women in all digit ratios except 2D:3D and R2D:4D (P<0.05). The sex difference in 3D:5D was the most significant. (2) When grouped by major, there were significant differences between PE major and non-PE major in all digit ratios except 2D:3D and R2D:4D (P<0.05). The difference in 3D:5D was the most significant. (3) There were no statistically significant differences in all digit ratios between left and right hands (P>0.05). It is speculated that the 5D related digit ratios were closely related to the sex and athletic ability.